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Sains
Malaysiana 53(7)(2024): 1617-1630
http://doi.org/10.17576/jsm-2024-5307-11
Antioxidant
Activities and Laxative Effect of Bioactive Compounds from Cynara cardunculus var. sylvestris
(Aktiviti
Antioksidan dan Kesan Laksatif Sebatian Bioaktif daripada Cynara cardunculus var. sylvestris)
FATMA TAJINI*, AWATEF JELASSI, AMAL
HAMDANI, ALI SALEM, OLA ABDELHEDI, ABID OUERGHUI & HICHEM SEBAI
Laboratory of Functional Physiology and Valorization of
Bio-Resources – Higher Institute of Biotechnology of Béja, University of
Jendouba, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Tunisia
Diserahkan: 19 Januari 2024/Diterima: 4 Jun 2024
Abstract
The present study aims to
investigate the antioxidant and anti-constipation activities of the derived
phenolic extracts in ribs wild cardoon (Cynara
sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited
high level of phenolic compounds and excellent antioxidant activities with
significant IC50 values (p
Keywords: Anti-constipation; Cynara cardunculus var. sylvestris; gastrointestinal motility; oxidative stress; phenolic compounds
Abstrak
Penyelidikan ini bertujuan untuk mengkaji
aktiviti antioksidan dan anti-sembelit daripada ekstrak fenol yang diperoleh
dalam kadun liar rusuk (Cynara sylvestris).
Ekstrak yang dianalisis (aseton, metanol dan akueus) menunjukkan tahap sebatian
fenol yang tinggi dan aktiviti antioksidan yang sangat baik dengan nilai IC50 yang ketara (p≤0.05), seperti yang dinilai oleh
aktiviti penangkapan radikal DPPH (0.84±0.08 - 2.44±0.007 μg/mL), ujian
radikal kationik ABTS (0.96 ±0.01- 136.67±6.75 μg/mL) dan ujian pelunturan
β-karotena (2.09±0.02-51.12±1.32 μg/mL). Juga, paras serat makanan
tidak larut yang lebih tinggi didapati (56.18 ± 0.91% DW serat detergen
neutral). Penyelidikan in vivo telah dijalankan ke atas tikus Wistar untuk mengkaji keupayaan
ekstrak akueus C. sylvestris (CSAE)
dalam peningkatan transit gastro usus
dan rawatan sembelit yang disebabkan oleh Loperamide (dikomersialkan sebagai
Idium). Peningkatan ketara sebanyak 15, 24.5 dan 32.4% dalam motiliti gastro
usus direkodkan apabila dos CSAE meningkat (masing-masing 75, 150 dan 300 mg/kg
b.w). Pengambilan makanan, penggunaan air, bilangan dan berat najis juga
dinaikkan secara kebergantungan dos, berbanding kawalan positif (tidak
dirawat). Tambahan pula CSAE memberikan perlindungan yang ketara (p≤0.05)
dan kebergantungan dos terhadap tekanan oksidatif dengan mengekalkan aktiviti
enzim antioksidan normal (SOD dan GPx) dalam usus dan kolon mukosa dan
menjadikan tahap enzim hepatik (AST dan ALT) dan buah pinggang (urea dan
kreatinin) kepada nilai normal. Keputusan ini boleh dijelaskan oleh banyaknya
sebatian fenol dan gentian tidak larut dalam tumbuhan ini. Oleh itu,
penggunaannya boleh digalakkan dalam pengaplikasian makanan dan farmaseutikal
sebagai makanan tambahan antioksidan dan laksatif.
Kata kunci: Anti-sembelit; Cynara cardunculus var. sylvestris; motiliti gastro usus;
sebatian fenol; tekanan oksidatif
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*Pengarang untuk surat-menyurat;
email: tajinifatma24@gmail.com
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